产纤溶酶米曲霉的筛选及诱变育种
来源:     发布日期:2020/06/09 22:56:10  浏览次数:

产纤溶酶米曲霉的筛选及诱变育种

Screening and Mutagenesis of the Fibrinolytic Enzyme Producing Strain Asperillus oryzae

DOI:

中文关键词: 纤溶酶 米曲霉 筛选 诱变

英文关键词: fibrinolytic enzyme,Asperillus Oryzae,screening,mutagenesis

基金项目:

高占争 赵允麟

江南大学教育部工业微生物重点实验室,江苏无锡214036

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中文摘要:

利用自己设计的筛选方法,从不同的酿造曲样中筛选出1株产纤溶酶酶活较高的米曲霉菌株SA-6,其产纤溶酶酶活为25U/mL。SA-6经过紫外线、^60Co和亚硝基胍诱变,筛选得到突变株NA-25,其产酶酶活为63U/mL,是出发菌株SA-6的2.52倍。突变株NA-25的发酵性状改变,其产纤溶酶的高峰比出发菌株迟了约10h。

英文摘要:

A Asperillus Oryzae strain,SA-6 was isolated with different screening methods and the fibrinolytic enzyme activity was 25 U/mL.A mutant was then isolated after SA-6 was treated with UV,~(60)Co and NTG and the relevant firbrinolytic enzyme activity was enh

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