MBR中厌氧氨氧化运行特性及微生态结构

Operation Characterictics and Microbial Community Distribution of Anammox in a Membrane Bioreactor

DOI：

中文关键词: 膜生物反应器 厌氧氨氧化 颗粒污泥 微生物种群分布

英文关键词: membrane bioreactor anaerobic ammonia oxidation granular sludge microbial community distribution

基金项目:国家973计划项目(2007CB714036);江苏省自然科学基金项目(BK2007022);江苏省太湖水专项项目(BS2007125)

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中文摘要:

研究了膜生物反应器(MBR)中厌氧氨氧化(Anammox)的运行特性与微生态结构变化。采用含氮模拟废水进行试验,最终获得粒径集中在0.2~1 mm的红褐色厌氧氨氧化颗粒污泥。运行结果显示,厌氧氨氧化菌能够承受的容积负荷达0.245 kgTN/(m3.d);总氮、NH4+-N和NO-2-N去除率分别达到80%、81%、91%。水力负荷冲击试验表明,当HRT从14 h下降到7.9h时,NH4+-N、NO-2-N去除率相对稳定,分别保持在75%和85%左右,厌氧氨氧化仍然能够稳定进行。通过末端限制性酶切片断长度多态性(T-RFLP)试验发现,反应器运行完成后微生物呈多态性分布,优势菌突出,其中起Anammox作用的菌属主要为planctomyce、pirellula、gemmata、pseudomonas,这为厌氧氨氧化运行过程中微生物群落结构变化提供理论依据。

英文摘要:

The operation characteristics and microbial community distribution of Anammox process in the MBR was studied in this manuscript.Seeding with synthetic media,we found that the granular sludge with 0.2~1 mm diameter was obtained successfully.The nitrogen-loading rate amounted to 0.245 kgTN/(m3·d),and the removal efficiency of total nitrogen,NH4+-N and NO2——N was 80%,81% and 91 %,respectively.Experiencing the hydraulic shock text,the HRT of MBR was gradually shorted from 14 to 7.9 h,the NH4+-N and NO2——N removal efficiencies were kept at about 75% and 85%,respectively.Based on terminal restriction fragment length polymorphism(T-RFLP) analysis,Microbial community diversity was achieved,and the predominant populations of Anammox bacteria found in MBR system were the planctomyce,pirellula,gemmata,pseudomonas after enrichment.The results presented here provided a theoretical basis for the change of microbial community during Anammox process.

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