高效液相色谱法测定烟草底料中低聚糖的含量

Determination of Oligosaccharides in Tobacco Casing Materials by HPLC

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中文关键词: 高效液相色谱 烟草底料 低聚糖

英文关键词: HPLC tobacco casing materials oligosaccharide

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中文摘要:

建立了一种检测烟草底料中低聚糖(水苏糖、棉籽糖、蔗糖)的高效液相色谱分析方法。采用Waters Sugar Pak1钙型阳离子交换色谱柱(6.5mm×300mm,5μm);流动相为纯水;采用示差折光检测器进行检测;流量1.0 mL/min;柱温85℃;外标法定量。结果显示:3种糖的线性关系良好,相关系数在0.9991~0.9997之间;精密度、稳定性的相对标准偏差(n=5)都低于4.7%;平均回收率为96.9%~103.5%;检出限为3.5~4.0μg/mL。该方法前处理简单、快速,应用本方法可测定烟草底料中水苏糖、棉籽糖和蔗糖的含量变化。

英文摘要:

In this study,a method for determining the content of oligosaccharides,including sucrose,raffinose,stachyose in tobacco casing materials by high performance liquid chromatography(HPLC) was developed.Separation and quantification were achieved by Waters Sugar Pak1(6.5 mm 300 mm,5 μm) with 1.0 mL/min pure water as the mobile phase,column temperature at 85℃ and Refractive Index Detector.The quantified analysis of external standard calibration curves was used.There were good linear correlations between the concentrations of three sugars and their chromatographic peak areas and the correlation coefficients were 0.9991-0.9997.The recoveries were 96.9%-103.5%,the RSD of the method was less than 4.7%.And the detection limits were 3.5-4.0 g/mL.This method is simple,rapid and accurate,and has been applied for eh quantitative analysis of sucrose,raffinose,stachyose in tobacco casing materials.

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