粘细菌AHB103-1发酵生产抗肿瘤活性物质

Study on the Production of Antitumor Metabolite by Myxobacteria AHB 103-1

DOI：

中文关键词: 培养基 发酵工艺条件 抗肿瘤活性物质

英文关键词: medium fermentation conditions antitumor secondary metabolite

基金项目:国家863计划项目(2007AA021501)

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中文摘要:

研究了培养基成分和发酵工艺条件对AHB103-1产生抗肿瘤活性物质的影响。结果表明,较优培养基为:碳源为玉米淀粉;氮源为酵母粉;碳氮比为12∶6;培养基中添加5 mg/L的Gly、Glu和Ala;10 mg/L的Try和Tyr;1.0 mg/L的LiSO4、MnCl2、Na2MoO4和NaH2PO4对抗肿瘤活性物质的生成有明显的促进作用。优化后的发酵工艺条件为:发酵周期7 d,起始pH 7.4,发酵温度30℃;接种种龄4 d;500 mL摇瓶装液量125 mL;摇床转速150 r/min。

英文摘要:

This study investigated the effect of medium compositions and the environmental conditions on the production of antitumor metabolite and listed as follows: carbon source,corn starch,nitrogen source,yeast extract;the ratio of carbon and nitrogen,12:6;If 5 mg/L Gly,Glu,Ala,10 mg/L Try,Tyr,1.0 mg/L LiSO4、MnCl2、Na2MoO4、NaH2PO4 were supplemented to the culture broth,the yield of antitumor secondary metabolite would be significant improved.The optimum fermentation conditions were as follows: fermentation term,7 d;initiative pH 7.4;fermentation temperature,30 ℃;seed culture term,4 d;the volume of culture liquid contained in 500 mL conical tank,125 mL;rotor speed,150 r/min.

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