研究了植物生长调节剂对Metarhizium anisopliae Tax-27产紫杉醇的影响,采用HPLC法检测。结果表明,赤霉素(GA3)质量浓度为4mg/L、茉莉酸甲酯(MeJA)质量浓度为60umol/L、矮壮素(CCC)质量浓度为25mg/L、6-苄氨基嘌呤(6-BA)质量浓度为4mg/L、萘乙酸(NAA)质量浓度为4 mg/L时该菌产紫杉醇的质量浓度分别为:670.49、838.11、942.57、455.72、450.48、953.35μg/L。但GA3、MeJA和CCC对M.anisopliae Tax-27菌丝体的生长有明显的抑制作用;6-BA和NAA对菌丝体的生长均有促进作用。
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In this study,the effect of plant growth regulators on the taxol biosynthesis by Metarhizium anisopliae Tax-27 was investigated.The results showed that the titer of taxol was 670.49 μg/L,838.11μg/L,942.57μg/L,455.72 μg/L,450.48 μg/L when 4 mg/L gibberellin(GA3),60μmol/L methyl jasmonate(MeJA),25mg/L chlormequat chloride(CCC),4 mg/L 6-benzylaminopurine(6-BA),and 4 mg/L 1-naphthlcetic acid(NAA) was feed to the fermentation broth,respectively..However,the addition of GA3,MeJA,CCC inhibited the mycelial growth of M.anisopliae Tax-27,on the other hand,the addition of 6-BA and NAA significance increased the mycelia growth.
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