食品模拟物中双酚A迁移量的间接竞争免疫PCR检测方法

Research of Indirect Immuno-PCR Detection for Migration of BPA in Food Simulants

DOI：10.3969/j.issn.1673-1689.2014.09.008

中文关键词: 免疫传感 双酚A 迁移 食品模拟物

英文关键词: Immuno-PCR, BPA, Migration, Food Simulants

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中文摘要:

通过抗原-抗体的特异性反应，结合荧光PCR，建立了食品接触材料中双酚A在食品模拟物中迁移量的快速、灵敏的间接竞争免疫PCR检测方法。通过优化包被抗原浓度、探针DNA的量、抗体质量浓度等一系列条件，双酚A的线性质量分数范围在0.01~10 pg/g之间 ，检测低限为3.0 fg/g，4种食品模拟物中双酚A 的添加回收率在82.8%~115.2%，该方法特异性好、灵敏度高，适用于食品接触材料中双酚A及其他小分子化学物的痕量迁移检测，为其迁移行为及迁移模型的建立提供了技术支撑。

英文摘要:

Combine fluorescence PCR and specific antigen-antibody reaction, a rapid and sensitive detection method BPA in food contact materials was developed. After the optimization of the coating antigen concentration, the amounts of probe DNA, and the antibody concentration, BPA detection limit was low as 3.0 fg/g, and the linear concentration range was 0.01~10 pg/g. The recoveries of fruit drinks were 82.8%~115.2%. This method was specific, sensitive, and suitable for trace migration detection research of BPA and other small molecule chemicals.

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