蛋白酶复合水解文蛤肉制备海鲜香料的工艺
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蛋白酶复合水解文蛤肉制备海鲜香料的工艺

Preparation of Seafood Seasoning by Complex Enzymolysis of Meretrix lusoria L. Meat

DOI:10.3969/j.issn.1673-1689.2014.09.009

中文关键词: 文蛤 酶解 蛋白酶 感官评价 海鲜

英文关键词: Meretrix lusoria L., enzymolysis, protease, sensory evaluation, seafood

基金项目:

作者

单位

高原

江南大学 药学院江苏 无锡 214122

陆震鸣

江南大学 药学院江苏 无锡 214122

李恒

江南大学 药学院江苏 无锡 214122

钱建瑛

江南大学 药学院江苏 无锡 214122

史劲松

江南大学 药学院江苏 无锡 214122

许正宏

江南大学 药学院江苏 无锡 214122

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中文摘要:

以感官评分为指标,比较了不同种类蛋白酶和用酶量对文蛤肉水解液风味的影响,并采用正交实验优化了文蛤肉二段法复合酶解工艺条件:复合蛋白酶75 U/g,中性蛋白酶75 U/g,在45 ℃、自然pH下水解3 h;然后加入风味蛋白酶50 U/g,在50 ℃、自然pH下水解1 h。最终获得的水解液色泽为棕黄色,具有咸鲜滋味和明显蛤类特征香气,感官评分为5.5分,味精当量EUC高达53.25 g/L,氨基氮含量为4.16 g/L,水解度为35.3%,水解得率为36.4%,多肽含量为21.8 g/L。

英文摘要:

Based on sensory scores, effects of category and concentration of protease on the enzymolysis of Meretrix lusoria L. meat were studied. Orthogonal experiment was used to obtain the optimal two-step complex enzymolysis condition of Meretrix lusoria L. meat. The meat was hydrolyzed with compound protease(75 U/g) and neutral protease(75 U/g) at 45 ℃ and natural pH for 3 h; then flavor protease(50 U/g) at 50 ℃ and natural pH for 1 h. The Meretrix lusoria L. hydrolysate possessed light yellow color, fresh salty flavor, and clam characteristic aroma. The sensory score, EUC, amino nitrogen content, degree of hydrolysis(DH), enzymatic hydrolysis yield of the Meretrix lusoria L. hydrolysate and content of peptides were 5.5, 53.25 g MSG/L , 4.16 g/L, 35.3%, 36.4% and 21.8 g/L, respectively.

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