Bacillus pumilusHN-10对Trichothecium roseum的拮抗作用及菌体结构的影响

Effect ofBacillus pumilusHN-10 on Antagonistic Activity and Cell Structure ofTrichothecium roseum

DOI：10.3969/j.issn.1673-1689.2019.06.004

中文关键词: 粉红单端孢 短小芽孢杆菌 拮抗作用 抑菌机理

英文关键词: Trichothecium roseum,Bacillus pumilusHN-10,antimicrobial activity,antifungal mechanism

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中文摘要:

探究一株分离自腐败马铃薯湿粉条的短小芽孢杆菌（Bacillus pumilusHN-10）对粉红单端孢（Trichothecium roseum）的拮抗作用及菌体结构的影响.采用体外拮抗法检测其对T.roseum菌丝体生长和孢子萌发的影响，结合甜瓜（Cucumis melo L）损伤接种试验验证其对病害控制效果，测定了供试病原菌粉红单端孢的生长曲线，电导率，胞内蛋白和核酸渗出量等生理，生化指标，并借助扫描电镜图像来进一步考察拮抗效果.结果表明：短小芽孢杆菌（B.pumilusHN-10）发酵液对T.roseum菌丝生长和孢子萌发有明显抑制效果，发酵液对接种粉红单端孢的甜瓜引起的腐烂抑制效果显著，且随发酵液浓度越大，伤口腐烂病斑直径越小；发酵液蛋白粗提物能够抑制T.roseum菌体生物量增加；电导率、吸光度（OD260，OD280）及扫描电镜结果发现，该粗提物处理改变或破坏了细胞膜结构完整性，提高了细胞膜的通透性，使胞内物质渗出.

英文摘要:

A strain isolated from the decayed potato vermicelli，which was identified asBacillus pumilusHN-10. In this test，the effect ofBacillus pumilusHN-10 on antagonistic activity and cell structure ofTrichothecium roseumwas studied. In vitro antifungal activity ofB pumilusHN-10 was determined through the mycelial growth and spore germination of T roseum. Meanwhile，the disease control effect was verified in melon（Cucumis melo L） fruits inoculated with pores treated with B pumilus HN-10 fermentation fluid. Furthermore，a series of physiology and biochemistry indexes，which including growth curve，conductivity，the exudation content of Intracellular protein and nucleic acid etc，and combined with scanning electron microscope（SEM） observation，were measured to elucidate the antagonistic effect against the pathogen. The results showed thatB pumilusHN-10 fermentation fluid onT.roseummycelial growth and spore germination had a significantly inhibiting effect. The fermentation fluid had a great inhibitory effect for the decay of T.roseum，and companing with the higher fermentation fluid concentration，the diameter of the wound rot changed smaller. The growth curve ofT.roseumimplied that B pumilus HN-10 fermentation fluid crude protein extract had an obvious inhibition effect onT.roseumbiomass. In addition，the fermentation fluid crude extract could change or destroy the structural integrity of cell membrane ofT.roseumand significantly enhance the permeability of cell membrane，which led to leakage of the intracellular contents.

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