聚乙烯醇双酶降解工艺条件优化

Optimization of Two-Step Enzymatic Degradation of Polyvinyl Alcohol

DOI：10.3969/j.issn.1673-1689.2019.06.005

中文关键词: 聚乙烯醇 PVA脱氢酶 氧化型PVA水解酶 Box-Behnken实验 响应面法

英文关键词: polyvinyl alcohol,polyvinyl alcohol dehydrogenase,oxidized polyvinyl alcohol hydrolase,Box-Behnken,response surface methodology

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中文摘要:

作为纺织和造纸工业中的重要污染物，聚乙烯醇（PVA）的高效降解广受关注。为提高PVA的降解效率，本研究中对PVA脱氢酶（PVADH）和氧化型PVA水解酶（OPH）降解PVA的工艺条件进行了优化。通过Box-Behnken实验和响应面分析，确定PVA双酶降解的反应条件为：PVA 1 g/L，PVADH 123 U/mL，OPH 12 U/mL，pH 7.7，酶解温度41 ℃，Ca2+浓度 1 mmol/L，PQQ浓度 6 μmol/L 。在该条件下PVADH和OPH共同催化4 h，PVA降解率达到95%以上。上述结果表明，PVADH和OPH双酶能有效降解PVA。研究结果将促进酶法降解PVA的工业化应用。

英文摘要:

As one of the most important pollutants in textile and paper industries，polyvinyl alcohol（PVA） has caused much attention. To improve the degradation efficiency，the reaction conditions of PVA degradation by using polyvinyl alcohol dehydrogenase（PVADH） and oxidized polyvinyl alcohol hydrolase（OPH） were optimized. Based on the Box-Behnken design and response surface strategy，optimal conditions were set as follows：PVA 1 g/L，PVADH 123 U/mL，OPH 12 U/mL，pH 7.7，reaction temperature at 41 ℃，Ca2+ 1 mmol/L，PQQ 6 μmol/L. Under these conditions，the degradation efficiency can reach over 95% after PVADH and OPH 4 h reaction. These results indicated that PVADH and OPH can co-degrade PVA efficiently，which will promote industrial applications of enzymatic degradation of PVA.

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