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固态发酵废菌棒高产哈茨木霉孢子
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Solid-State Fermentation ofTrichoderma harzianumfor High Yield Spore with Waste Bacteria Stick
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DOI:10.3969/j.issn.1673-1689.2019.06.003
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中文关键词: 孢子 生防剂 废菌棒 优化 固态发酵
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英文关键词: spore,biocontrol agent,waste bacteria stick,optimization,solid-state fermentation
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基金项目:
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摘要点击次数: 31
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全文下载次数: 22
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中文摘要:
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以废菌棒为固态发酵主要基质,采用单因素和响应面分析法(RSM)优化哈茨木霉固态发酵生产孢子,最后进行固态发酵放大实验。结果显示:最佳培养条件为接种量(质量浓度)7.5 g/dL,装料量20 g,pH自然,培养温度28 ℃,发酵周期144 h;响应面分析法优化的理论值为:初始含水质量分数57.07%,淀粉质量分数3.08%,NaNO3质量分数1.99%,理论孢子产量5.65×109 个/g,验证实验结果5.91×109个/g,符合理论预测;使用10 L自制固态发酵罐进行放大实验,通风条件下孢子产量显著提高,达到8.20×109个/g。
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英文摘要:
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The solid-state fermentation ofTrichoderma harzianum,in which waste bacteria stick were used as substrate,was optimized by using single-factor test and response surface methodology(RSM). The up-scaled solid-state fermentation was carried out. Results showed that the optimal conditions were:inoculation 7.5 g/dL(w/w),substrate loading quantity 20 g,natural pH,culture temperature 28 ℃ and fermentation time 144 h. The optimal conditions of response surface analysis method were 57.07%(w/w) initial water content,3.08% starch and 1.99% NaNO3. The theoretical spore yield was 5.65×109 cfu/g compared with the actual yield 5.91×109 cfu/g,which was consistent with the theoretical prediction. The eventual sporulation was 8.20×109 cfu/g at the condition of ventilation by using the 10 L solid-state fermenter.
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