紫芝子实体活性多糖的纯化和结构解析

Purification and Structure Elucidation of Bioactive Polysaccharide from the Fruiting Bodies ofGanoderma sinense

DOI：10.3969/j.issn.1673-1689.2019.01.020

中文关键词: β-葡聚糖 分离纯化 活性 多糖

英文关键词: β-glucan,isolation and purification,activity,polysaccharide

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中文摘要:

以刺激RAW264.7细胞释放NO活性模型为指导，通过乙醇沉淀和DEAE阴离子交换柱层析，从紫芝子实体中分离得到均一多糖GS30S1-20E，采用红外光谱、核磁共振、气相色谱-质谱法和高效液相等现代仪器，研究了GS30S1-20E的结构特性。结果表明：GS30S1-20E是一种β-D-葡聚糖，其重均相对分子质量为2.418×106，数均相对分子质量为1.668×106，多分散指数为1.450，特性粘度为927.49 mL/g，以β-（1，3）-糖苷键为主链和β-（1，6）-糖苷键为支链，主链与支链上糖残基的摩尔比为3∶1，具有刺激巨噬细胞释放NO的活性。

英文摘要:

Crude polysaccharide GS30 precipitated from hot water extracts of the fruiting bodies ofGanoderma sinensewere separated by DEAE-column chromatography，and then treated with ethanol precipitation to get a purified polysaccharide fraction GS30S1-20E. Modern analytical techniques，such as IR，NMR，GC/MS and HPSEC were conducted to analyze the structural characteristics of GS30S1-20E. Results showed that GS30S1-20E was a β-D-glucan with a high weight average molecular mass of 2.418×106 and number average molecular weight of 1.668×106，polydispersity of 1.450 and large viscosity of 927.49 mL/g. GS30S1-20E contains a backbone of（1→3）-linked-β-D-glucopyranosyl residues，bearing branches at the O-6 position of every three sugar residues along the backbone. An in vitro immunomodulating activity assay revealed that GS30S1-20E could significantly increase the release of NO from RAW 264.7 cells.

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