两种多糖对齐口裂腹鱼免疫性能的影响

Effect of Two Kinds of Polysaccharide on Immune Performance ofSchizothorax prenanti

DOI：10.3969/j.issn.1673-1689.2019.01.021

中文关键词: 氧化魔芋葡甘露聚糖 氧化魔芋葡甘露聚糖硫酸酯 齐口裂腹鱼 超氧化物歧化酶 髓样分化因子 干扰素影响因子

英文关键词: oxidized konjac glucomannan,oxidized konjac glucomannan sulfates,Schizothorax prenanti,SOD,myeloid differentiation factor,interferon regulator factor

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中文摘要:

探讨OKGM、OKGMS两种多糖对齐口裂腹鱼免疫性能的影响，将齐口裂腹鱼随机分为7组，两种多糖添加剂量分别为质量分数0.4%、0.8%、1.6%，饲喂60 d，测定齐口裂腹鱼血清免疫及抗氧化指标，并采用RT-PCR技术检测组织MyD88、IRF7基因相对表达量。结果表明，OKGMS1.6组能显著提高齐口裂腹鱼血清IgM活性、ACH50含量；OKGMS0.4、OKGMS0.8、OKGM0.4、OKGM0.8组均能显著降低血清MDA的含量；OKGMS与OKGM均能提高血清SOD活性；OKGMS能上调齐口裂腹鱼肠道MyD88基因mRNA相对表达量及肝脏、脾脏、中肾IRF7基因mRNA相对表达量；OKGM能上调肠道、头肾、中肾MyD88基因mRNA相对表达量及肠道、肝脏、脾脏、中肾IRF7基因mRNA相对表达量。结果表明：适量添加OKGM、OKGMS能提高齐口裂腹鱼抗氧化活性及免疫性能。

英文摘要:

The experiment was conducted to investigate the effects of oxidized konjac glucomannan and oxidized konjac glucomannan sulfates on immune performance ofSchizothorax prenanti. TheSchizothorax prenantiwere randomly divided into 7 groups and each group included 3 replicates of 20 fish. The experiment lasted for 60 days with the treatment groups were contained 0.4%、0.8%、1.6% of OKGM and OKGMS. And body composition and serum biochemical indices were measured and MyD88、IRF7 mRNA level in tissue were determined by RT-PCR. Results：Content of IgM、ACH50 of OKGMS1.6 were significantly more than the control group；The content of MDA was significantly lower of group OKGMS0.4、OKGMS0.8、OKGM0.4、OKGM0.8 than the control group；OKGMS、OKGM both increased the activity of SOD；OKGMS could up-regulate MyD88 mRNA level in gut and IRF7 mRNA level in liver、spleen and mesonephros；OKGM could increase MyD88 mRNA level in gut、headkidney and mesonephros and IRF7 mRNA level in gut、liver、spleen and mesonephros. Conclusions：Add the appropriate amount of OKGM and OKGMS could increase the antioxidant activity and immune functions ofSchizothorax prenanti.

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