基于适配体识别的侧向层析法快速检测单核细胞增生李斯特氏菌

Aptamer-Based Lateral Flow Strip for Rapid Detection of Listeria monocytogenes

DOI：10.3969/j.issn.1673-1689.2020.04.012

中文关键词: 核酸适配体，食源性致病菌，单核细胞增生李斯特氏菌，试纸条

英文关键词: aptamer,food-borne pathogen,Listeria monocytogenes,lateral flow strip

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中文摘要:

构建了一种快速检测单核细胞增生李斯特氏菌的适配体试纸条。以单核细胞增生李斯特氏菌核酸适配体为识别分子，纳米金为显色信号，基于双适配体夹心原理，制备了用于检测食品中单核细胞增生李斯特氏菌的适配体试纸条，并对制备条件如适配体浓度、胶体金溶液pH值、纳米金-适配体复合物和链霉亲和素-纳米金复合物的包被量等进行了优化。结果显示，在优化条件下，该试纸条对单核细胞增生李斯特氏菌的裸眼可视检测限为103 cfu/mL，且具有很高特异性。可实现样品中单核细胞增生李斯特氏菌的快速定性检测。

英文摘要:

An aptamer-based lateral flow strip was developed for the rapid detection ofListeria monocytogenes. This assay was designed based on the sandwich-type format by using primary aptamer conjugated with gold nanoparticles（AuNP） as a signaling probe and secondary aptamer coated membrane as a capturing probe. Several experimental conditions were investigated，including the concentration of aptamer，pH of AuNP solution，and loading quantity of AuNP-aptamer conjugates and streptavidin-aptamer conjugates. Under optimized conditions，the aptamer-based lateral flow strip showed a visual limit of detection（LOD） of 103 cfu/mL，and had no significant cross-reactivity with several food-borne pathogens. The aptamer-based lateral flow strip was shown to provide a reliable，accurate method for rapid detection ofListeria monocytogenesin the real samples.

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