安化黑茶对载脂蛋白E敲除小鼠动脉粥样硬化的影响

Effect of Anhua Dark Tea on Atherosclerosis in Apolipoprotein E Knockout Mice

DOI：10.3969/j.issn.1673-1689.2020.04.011

中文关键词: 安化黑茶 载脂蛋白E敲除 白细胞介素-1β 单核细胞趋化蛋白-1 氧化型低密度脂蛋白 肿瘤坏死因子α 高敏感性C-反应蛋白

英文关键词: Anhua dark tea,APOE-/-,IL-1β,MCP-1,OX-LDL,TNF-α,hs-CRP

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中文摘要:

观察安化黑茶对载脂蛋白E敲除（APOE-/-）小鼠血清中血脂含量、氧化型低密度脂蛋白（OX-LDL）及相关炎症因子的影响，推测其干预AS的相关机理。选取8周龄雄性APOE-/-小鼠55只，持续西方饮食饲料喂养13周，同时选用8周龄的C57小鼠作为空白对照予以普通饲料喂养13周，13周后随机抽取5只行HE染色，确定造模成功后根据体质量分层，然后随机分为模型组、阿托伐他汀组和安化黑茶高、中、低剂量组。造模成功的APOE-/-小鼠继续西方饮食饲料喂养8周，阿托伐他汀组予以10 mg/（kg·d）药物进行灌胃干预，安化黑茶高、中、低剂量组分别予以2.16、1.44、0.72 g/（kg·d）的剂量灌胃干预，空白组和模型组予以等剂量的生理盐水灌胃干预，以上药物连续干预8周。实验末各组小鼠摘眼球取血清用于血脂四项和相关炎症因子的指标检测，称取小鼠体质量、肝质量、腹部脂肪质量，计算肝指数；取小鼠主动脉用于HE染色形态学观察。阿托伐他汀和安化黑茶不同剂量组小鼠主动脉瓣膜附近的斑块显著低于模型组（P

英文摘要:

The study aimed to observe the effect of Anhua dark tea on serum lipids，OX-LDL and related inflammatory factors in APOE-/- mice to speculate its anti-AS mechanism. Fifty-five eight-week-old male APOE-/- mice were fed with western diet for 13 weeks continuously. Ten eight-week-old male C57 mice as normal group were fed with nomal diet for 13 weeks continuously. After 13 weeks，five mice were randomly selected for HE staining to determine the model. Then the mouse were randomly divided into model group，atorvastatin group and high，medium and low doses of Anhua dark tea groups. They were given saline，atorvastatin and Anhua dark tea with doses of 10, 2.16, 1.44 and 0.72 g/（kg·d） by gavage, respectively. The blank group were given equal doses of saline by gavage and the APOE-/- mice continued to be fed with western diet for 8 weeks. At the end of experiment，the eyeball was taken for blood and the serum was separate for blood lipids and related inflammatory factors.The body，liver，spleen，and abdominal fat of the mice were weighed，and the liver index was calculated. The aorta of the mice were taken for HE staining. Compared with the model group，the aortic root plaques， the weights of body，liver，spleen，abdominal fat and liver index，the levels of TG，IL-1β，MCP-1，OX-LDL，TNF-α，hs-CRP of Anhua dark tea groups were decreased significantly（P

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