一测多评法测定6种大豆异黄酮的质量分数

Quantitative Analysis of Six Soybean Isoflavones by QAMS

DOI：10.3969/j.issn.1673-1689.2020.04.013

中文关键词: 大豆异黄酮 一测多评 相对校正因子 高效液相色谱

英文关键词: soybean isoflavone,quantitative analysis of multi-components by single-marker（QAMS）,relative correction factor（RCF）,high performance liquid chromatography

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中文摘要:

基于高效液相色谱紫外双波长检测方法，建立只用一个对照品同时测定6种大豆异黄酮含量的一测多评方法，以降低食品中大豆异黄酮含量测定的分析成本并提高分析效率。样品经甲醇超声波辅助提取后，采用C18反相色谱柱；柱温25 ℃；进样量10 μL；检测波长262 nm和248 nm；流量1.0 mL/min；以体积分数0.2%甲酸水溶液和甲醇运行洗脱。通过一测多评分析确定6种大豆异黄酮的定量标准曲线、线性范围、相对校正因子、相对保留时间及相对峰面积，并对检测结果的精密度、稳定性、重复性、加样回收率以及系统的耐用性和适应性进行验证，且与外标法实验结果相比对，6种大豆异黄酮在检测范围内均呈现出良好的线性关系（R²＞ 0.999 9）。

英文摘要:

The study aimed to establish a method for quantitative analysis of multi-components by single-marker (QAMS) to quantify six soybean isoflavones by HPLC with ultraviolet detection of dual wavelength，which would decrease the cost of quantitative analysis of isoflavones and enhance the efficiency. The sample was extracted by methanol under the ultrasonic wave assistance，then analyzed by HPLC with a C18 column. The running condition was 25 ℃，injection volume of 10 μL，detection wavelength of 262 nm and 248 nm，elution rate of 1.0 mL/min with 0.2% formic acid in water and methanol. Six soybean isoflavones were used for the QAMS to obtain the standard curves，linear ranges，relative correction factors (RCF)，relative retention times，and relative peak areas. The precision，stability，repeatability，sample recovery rate，system durability and adaptability of QAMS method were tested and compared with those of external standard method. The results showed that six soybean isoflavones had good linearity within the analysis range (R²> 0.999 9).

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