直接酶联免疫吸附法检测猪血清IgG的影响因素
Investigation and Optimization of Factors on Measuring IgG in Porcine Serum by Direct Enzyme-Linked Immunoserbent Assay
DOI:
中文关键词: 直接酶联免疫吸附法 G型免疫球蛋白(IgG) 猪血清
英文关键词: Direct Enzyme-Linked Immunoserbent Assay,Immunoglobulin G(IgG),Porcine Serum
基金项目:
罗磊 丁霄霖
江南大学食品学院,江苏无锡214036
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中文摘要:
直接酶联免疫吸附法检测猪血清中G型免疫球蛋白(IgG),最小检测量为10ng,最适检测范围纯IgG为10~60ng,猪血清IgG为10~40ng。酶标抗体最适稀释度为1/3500。酶标板内与酶标板间平行测定变异系数均小于5%。
英文摘要:
This paper studied the method of measuring Immunoglobulin G in porcine serum by Direct Enzyme-Linked Immunoserbent Assay.ELISA's smallest measurement limit was 10 ng.The best measurement ranges were 10~60 ng for pure IgG and 10~40 ng for porcine serum IgG
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