青木香发酵后总马兜铃酸和马兜铃酸Ⅰ的含量测定

Determination of Total Aristolochic Acids and Aristolochic Acid Ⅰ in Radix aristolochiae after Fermentation

DOI：

中文关键词: 青木香 真菌发酵 总马兜铃酸 马兜铃酸Ⅰ 含量测定

英文关键词: Radix aristolochiae fungi fermentation total aristolochic acids aristolochic acid Ⅰ determination

基金项目:江苏省教育厅自然科学基金项目(06KJB360083);江苏省“青蓝工程”项目(2008)

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中文摘要:

以灵芝、槐耳等20个真菌为菌种、青木香药材为基质,运用固体发酵技术,在一定的条件下进行发酵试验,筛选出13个菌种能在青木香药材上生长良好,表明这些菌种能与药材形成较好的发酵组合形成发酵品。采用反相HPLC二元梯度洗脱法测定13种青木香发酵品中马兜铃酸Ⅰ(AAⅠ)的含量及紫外分光光度法(UV)测定这些发酵品中总马兜铃酸(TAA)含量。HPLC测定表明,13种青木香发酵品主要肾毒性成分马兜铃酸Ⅰ均有不同程度的下降,其中有6种发酵品的马兜铃酸Ⅰ的下降率在50%以上;UV测定发现,这些发酵品中总马兜铃酸含量均有所下降,下降率最高者为46.76%,最低者为7.61%;HPLC和UV综合(加权)分析表明,13种真菌(均以代号表示)对青木香肾毒性成分的降解能力由大至小依次排列如下:F-1>F-2>F-5>F-6>F-8>F-13>F-4>F-11>F-3>F-9>F-10>F-12>F-7。提示不同真菌发酵可在不同程度上降低中药青木香中肾毒性成分马兜铃酸Ⅰ和总马兜铃酸的含量。

英文摘要:

With Radix aristolochiae as substrate,twenty fungi was test and 13 different fungi were found to be grown on it.Compare with that of the control,the AA Ⅰ content was decreased in those 13 fermentation product,and the AA Ⅰ content in 6 fermentation product was lower than 50%,compared with that of the control.Similarly,the TAA contents were also detected to be decrease.Among of them,the highest and lowest rate of decline was 46.76% and 7.61%,respectively.After statistical analysis,the ability to decompose the nephrotoxic substances in Radix aristolochiae of the 13 fungi was illustrated as follows(from the strong to the weak): F-1> F-2> F-5> F-6> F-8> F-13> F-4> F-11> F-3> F-9> F-10> F-12> F-7(the symbol of different fungi).Those results indicated that different fungi fermentation could efficient decrease the nephrotoxic components in Radix aristolochiae.

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