| 鸡枞菌菌丝体皂甙含量测定方法的研究
| Determination of Saponins from the Mycelia of Termitomyces albuminosus in Submerged Culture
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| DOI：
| 中文关键词: 鸡枞菌 菌丝体 皂甙 测定
| 英文关键词: Termitomyces albuminosus mycelia saponins determination
| 基金项目:国家863资助项目(2007AA021506);国家973资助项目(2007CB707800);教育部新世纪优秀人才支持计划(NCET20720380)
| 作者
| 单位
| 聂晓东
| 江南大学,医药学院,江苏,无锡,214122
江南大学,工业生物技术教育部重点实验室,江苏,无锡,214122
| 许泓瑜
| 江南大学,医药学院,江苏,无锡,214122
| 陆震鸣
| 江南大学,工业生物技术教育部重点实验室,江苏,无锡,214122
| 许正宏
| 江南大学,医药学院,江苏,无锡,214122
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| 中文摘要:
| 采用分光光度法测定鸡枞菌发酵菌丝体中皂甙含量。以人参皂甙Rg1为对照品,在吸收波长为550 nm下,标准浓度在10~240μg范围内,浓度与吸光度呈良好的线性关系,回归方程为:Y=0.003 0X-0.0405,R2=0.998 4。加样平均回收率为99.4%,相对标准偏差(RSD)为1.97%,精密度的RSD为1.89%,鸡枞菌丝体皂甙类含量为2.69 mg/g菌丝体。方法操作简单快速,灵敏度高且比较准确。
| 英文摘要:
| In this study,the spectrophotometric method was used for determining the content of saponins from the mycelia in submerged culture.Ginsenoside Rg1 as the standard,at 550 nm,the linear range of the saponins concentration was from 10 μg to 240 μg,and the regressive equation was Y= 0.003 0 X-0.040 5,R2=0.998 4.The recovery rate was 99.4% and the relative standard deviation(RSD) was 1.97%.The relative standard deviation(RSD) of the accuracy was 1.89%.The content of saponins from the mycelia in submerged culture was about 2.69 mg/g.The method presented here has been proven to be convenient to operate and the result is accurate and highly sensitive.
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