绿豆蛋白多肽对小鼠缺氧耐受力和免疫力提高的研究

Study of MBPP on Anoxia Endurance and Enhancing Immunity

DOI：

中文关键词: 绿豆蛋白多肽 缺氧耐受力 免疫力

英文关键词: mung bean protein or peptide(MBPP) Anoxia Endurance Enhancing immunity

基金项目:广东省科技厅社会发展重点计划项目(2008B030301015)

摘要点击次数: 264

全文下载次数: 776

中文摘要:

研究绿豆蛋白多肽(Mung bean protein,peptide MBPP)提高小鼠缺氧耐受力和免疫力的作用。采用高压凝胶色谱法测定MBPP均重分子量(Mw);并将小鼠分成3组连续灌胃给药30 d后,分别进行常压耐缺氧、亚硝酸钠中毒存活、和急性耐缺氧试验;采用ConA诱导小鼠淋巴细胞转化实验(MTT法)及迟发性变态反应(DTH)、抗体生成细胞(PFC)检测及半数溶血值(HC50)实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验,检测绿豆蛋白多肽对小鼠免疫功能的影响;其结果:绿豆蛋白多肽的Mw为195~1 000,其中195~510组分占70.2%以上;绿豆蛋白多肽0.82 g/kg.BW和1.23 g/kg.BW剂量组能明显延长小鼠常压缺氧存活时间(P

英文摘要:

To investigate the anoxia endurance and immunological of mung bean protein or peptide(MBPP) in mice.This research employs high performance gel permeation Chromatography(HPGPC)to investigate and evaluate the molecular weight’s(Mw)distribution of MBPP.The tested mice were randomly distributed into 3 groups,and were continuously given MBPP by intragastric administration for 30 days,then the living time of mice were measured in anoxia with normal air pressure,in poisoning by nitrite poisoning and in acute lack of blood in brain.The effect was evaluated on celluar immunity by ConA lymphocyte transformation test(MTT) and delayed type hypersensitivity(DTH).The impact of humoral immunity was detected by antibody-producing cells(PFC) test and the value of half of hemolysis(HC50) in mice.The non-specific immunity was detected by abdomen macrophages of mice devour chicken red cell test.The Mw MBPP is between 195~1000D,in which the component of 195-510D takes 70.2% or above.MBPP of 0.82 g/kg·BW and 1.23 g/kg·BW.dose group could significantly prolong the living time of mice with hypoxia(P

查看全文 查看/发表评论 下载PDF阅读器