免疫酶吸附法评价纳米金对抗体Fc端的吸附效果

A Study of Immunoenzymatic Techniques of the Adsorption Effect of Nano-Gold to Antibody-Fc Part

DOI：

中文关键词: 纳米金 胶体金 抗体Fc 免疫酶吸附技术 吸附

英文关键词: nano-gold colloidal gold antibody Fc part immunoenzymatic techniques adsorption

基金项目:国家“十一五”科技支撑计划(2006BAD04A00);天津市自然科学基金项目(06YFJMJC12600)

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中文摘要:

以简单、快速的微波技术制备出粒径约为15nm左右纳米金溶胶。为测定该纳米金对兔IgG抗体的吸附,扫描得到溶液吸附抗体前后的光吸收图谱,可以明显看到溶液最大光吸收峰从518 nm转移到526 nm,这种现象表明纳米金能够很好地结合抗体。为评价纳米金和抗体Fc端的吸附效果,以兔IgG抗体和酶标羊抗兔抗体反应后,测定上清液中酶标抗体对酶底物显色后O.D值的变化,结果表明,纳米金对抗体Fc端的吸附率在92%以上,证明该纳米金吸附主要是在抗体的Fc端。

英文摘要:

In this study,a nano-gold which diameters were about 15 nm was prepared through a the simple and rapid microwave technique.The nano-gold absorbing rabbit IgG was performed by UV-visible spectrophotometer.The absorption maximum wavelength of gold colloidal solution was obvious changed when IgG coupled to nano-gold.It range from 518 nm to 526 nm.For the adsorption effect of nano-gold to antibody-Fc part,it was described by the variation of O.D.When rabbit IgG reacted with HRP-labeled goat anti-rabbit IgG and formed a compound,the remaining HRP-labeled goat anti-rabbit IgG in the supernatant decreased after centrifugation.The HRP catalyzed substrate reaction with the color variation.Finally it was shown a good adsorption effect through the ratio of O.D,and the percentage was over 92%.

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