微波辅助法提取猕猴桃多糖的条件优化

Optimization of the Extraction of Polysaccharides with Microwave-Assisted Extraction from Actinidia chinensis

DOI：

中文关键词: 中华猕猴桃 多糖 微波辅助提取 均匀设计法

英文关键词: Actinidia Chinensis polysaccharide microwaveassisted extraction uniform experimental design method

基金项目:浙江省科技厅面上项目(2008C33031)

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中文摘要:

为了提高猕猴桃多糖的收率,研究了微波辅助提取法从中华猕猴桃根、茎和叶中分离多糖的过程,采用均匀设计法考察了固液质量体积比,提取时间和微波功率对多糖提取率的影响。结果发现不同原料的最佳提取条件也不同,根据试验数据和实际情况确定了3种多糖的最优提取条件分别为:根多糖的固液质量体积比为0.0169g/mL,微波功率为385W,作用时间24min;茎多糖的固液质量体积比为0.0170g/mL,微波功率为539W,作用时间为23.8min;叶多糖的固液质量体积比为0.0161g/mL,微波功率为539W,作用时间为23.5min。在最优提取条件下,根、茎、叶多糖的得率依次为7.0%、3.7%和3.2%,明显高于其它的提取方法。同时还经试验验证,3种多糖得率实验值与回归方程的计算值偏差均小于5%。

英文摘要:

Microwave-assisted extraction (MAE) was applied to the polysaccharides extraction from the dried root,stem and leaf of Actinidia Chinensis.Three key operation parameters,including the extraction time,the power of microwave and the ratio of material to solvent,were optimized with uniform experimental design method.The optimal conditions for extracting three raw materials were determined by the regression equations.Considering the efficiency and the feasibility of experiment,the actual operating conditions were 0.016 9 of the ratio of material to solvent,24 min of the extraction time and 385 W of the microwave power in the extraction of root;0.0170 of the ratio of material to solvent,23.8 min of the extraction time and 539 w of the microwave power in the extraction of stem;and 0.016 1 of the ratio of material to solvent,23.5 min of the extraction time and 539 W of the microwave power in the extraction of leaf,respectively.The extracted yields of polysaccharides in the actual optimal conditions were 7.0%,3.7%和3.2%.The deviation between the values from experiments and calculation from root and leaf were both below 5%,which demonstrated that the regression equation could predict the yield of polysaccharide in the given conditions well.

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