取代度相同的N-酰化低聚壳聚糖清除自由基作用的研究

The Radical Scavenging Effect of N-Acyl Chitosan Oligosaccharide

DOI：

中文关键词: 低聚壳聚糖 N-酰化 抗氧化 取代度

英文关键词: chitosan oligosaccharide N-acyl antioxidant activity substituting degree

基金项目:上海市教育委员会科研项目(07zz134);上海市重点学科建设项目专项基金(T1102);上海市生物医药和农业科技领域重点科技项目(08391911500);2009年上海市优秀学科带头人计划(09XD1402000)

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中文摘要:

低聚壳聚糖经N-酰化得到取代度相同的N-马来酰低聚壳聚糖和N-邻苯二甲酰低聚壳聚糖,其取代度均为0.25。用红外光谱对其结构进行表征,凝胶色谱测定其相对分子质量大小。并考察了其对羟基自由基(.OH)和1,1-二苯基苦基苯肼自由基(DPPH)的清除能力。结果表明:取代度相同,N-邻苯二甲酰低聚壳聚糖对.OH、DPPH的清除能力优于N-马来酰低聚壳聚糖。这说明取代度相同时,取代基的结构会影响N-酰化低聚壳聚糖对自由基的清除活性。

英文摘要:

N-maleyl chitosan oligosaccharide (NMCOS) and N-phthaloyl chitosan oligosaccharide (NPCOS) with the same substituting degrees (0.25) were synthesized.The structure were characterized by FTIR spectra,the molecular weight was measured by Gel Permeation Chromatog-raphy (GPC).Their antioxidant activity were evaluated by the scavenging of hydroxyl radical (.OH) and 1,1-Diphenyl-2-picrylhydrazyl radical ( DPPH).The results showed NPCOS have stronger hydroxyl and DPPH radical scavenging activity than that of NMCOS.The results presented here demonstrated that the antioxidant activity of N-acyl Chitosan Oligosaccharide with the same substituting degrees was determined by the substituting groups.

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