山楂叶和果中类SOD的提取与研究

Extraction and Characteristics of Superoxide Disutase from Hawthorn Leaves and Fruit

DOI：

中文关键词: 山楂叶 山楂果 SOD-L 抗氧化性

英文关键词: hawthorn leaves hawthorn fruit Superoxide disutase like Anti-oxidation

基金项目:辽宁省教育厅科学研究计划资助项目(200502)

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中文摘要:

实验对山楂叶、果中类SOD(Superoxide disutase like简称SOD-L)进行了提取与研究。通过连苯三酚自氧化法对山楂叶、果中SOD-L活性进行分析,测得山楂叶提取物中SOD-L的活力为630.14 u/mg;山楂果提取物中SOD-L的活力为252.05 u/mg;在280 nm有特征性吸收峰;在Mg2+和Cu2+的存在时SOD-L的活性有着不同程度的增强。SOD-L的最适温度在40℃;最适pH值在8.0。

英文摘要:

The manuscript described the extraction of the category SOD-L in hawthorn leaves and fruit.This experiment extract and analysis is through using pyrogallol since the oxidation of hawthorn in leaves and fruit category SOD-L,we measured extract of SOD-L vigor in hawthorn leaves and fruit was 630.14 u/mg and 252.05 u/mg,respectively.SOD-L in the bran has a typical absorption at 280 nm.And it was found that the activity of SOD increase with the presence of Mg2+,Cu2+.SOD-Lactivity of hawthorn in leaves and fruit in the bran had an obvious optimum stability at the condition of 40 ℃ and pH 8.0.

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