雄甾烯酮转化菌的诱变育种和发酵条件优化

Mutant and Optimization of Androstenone Translation Strain Mycobacterium sp. SH5

DOI：

中文关键词: 4烯--雄甾-3,17-二酮 l,4-二烯-雄甾-3,17-二酮 植物甾醇 生物转化

英文关键词: AD ADD phytosterol biotransformation

基金项目:北京联合大学重点学科建设项目(51102630517)

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中文摘要:

采用紫外线照射和NTG对实验室保存的一株对植物甾醇有降解能力的Mycobacterium sp. SH5进行诱变处理,得到一株雄甾-4-烯-3,17-二酮(Androst-4-end-3,17-dione,AD)和雄甾-1,4-二烯-3,17-二酮(Androst-1,4-end-3,17-dione,ADD)分解酶(9α-羟化酶)缺陷型菌株Mycobacterium sp.SH5-52。对Mycobacterium sp. SH5-52降解植物甾醇侧链得到AD(D)的培养基组成、接种量、温度、pH值和溶氧等发酵条件进行了研究。在最佳的发酵条件下,投料质量浓度为0.6 g/dL时,AD(D)的转化率由原来的37.2%提高到47.6%左右。

英文摘要:

Mycobacterium sp.SH5 was a potential strain for degrading phytosterol.After mutation by UV and NTG,a mutant with 9α-hydroxylase defective was selected and named as Mycobacterium sp.SH5-52.On the basis of mutation,the nutritional and environmental conditions of Mycobacterium sp.SH5-52 were investigated and optimized.With the optimum conditions,Mycobacterium sp.SH5-52 could degrade 0.6% substance phytosterol to Androst-4-end-3,17-dione(AD) and Androst-1,4-end-3,17-dione(ADD) with the average conversion ratio significantly improved from 37.2% to 47.6%.

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