花脸香蘑Lepista sordida LS7的鉴定及其发酵液抗菌活性分析

Identification of Lepista sordida LS7 and Antibiotic Activity Analysis of its Fermentation Broth

DOI：

中文关键词: 花脸香蘑 形态学 ITS 抗菌活性

英文关键词: Lepista sordida morphology ITS antibiotic activity

基金项目:国家自然科学基金项目(30771646)

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中文摘要:

对采集自青岛崂山的一株真菌LS7进行鉴定,通过对菌落形态和菌丝结构的形态学研究,以及对ITS和5.8s rDNA分子系统学分析,结果均说明此真菌为花脸香蘑(Lepista sordida)。采用不同有机溶剂对该菌株的发酵液萃取,对不同的萃取部位进行抗菌活性分析。通过对其发酵液的抗菌活性实验,发现该菌株的发酵液具有广泛的抗菌活性,对G~+、G~-细菌、酵母菌和霉菌都有很好的抑制作用,其中对植物致病菌黄瓜枯萎菌和辣椒炭疽菌效果明显。

英文摘要:

A strain LS7 was isolated from the Laoshan mountain of Qingdao.According to the morphological,cultural characteristics,and ITS and 5.8s rDNA sequence analysis,the isolate was identified as Lepista sordida.The fermentation broth was extracted by different organic reagents,and their antibiotic activity was also evulated in this study.This strain exhibited the inhibitory action on Gram-positive bacteria,Gram-negative bacteria and fungi,especially for Fusarium oxysporum and Colletotrichum capsicibutl.The results shown here provided the theoretical basis for the application of Lepista sordida in food and medicine industry.

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