Paecilomyces sp. S152胞外漆酶的合成调控

Improvement of Extracellular Laccase Production by Paecilomyces sp. S152

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中文关键词: 拟青霉 胞外漆酶 营养条件 同工酶 分布 表面活性剂

英文关键词: Paecilomyces sp. extracellular laccase nutrition conditions isozyme location surfactant

基金项目:国家杰出青年基金项目(20625619);教育部新世纪优秀人才支持计划项目(NCET-07-0378,NCET-05-0488)

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中文摘要:

探索了不同碳氮源营养条件下,Paecilomyces sp.S152胞外漆酶的分泌情况,发现麦芽糖(20 g/L)、大豆蛋白胨(10 g/L)及酵母粉(1 g/L)最适于Paecilomyces sp.S152胞外漆酶的合成,发酵7～8 d后,漆酶酶活达到5064 U/L。研究发现Paecilomyces sp.S152漆酶在细胞内及细胞外均有分布,添加1.5 g/L的Tween 80可促进胞内漆酶的释放,提高胞外漆酶酶活,缩短发酵周期(6 d),生产强度提高至946 U/(L·d)。

英文摘要:

The culture medium containing maltose(20 g/L),bean peptone(10 g/L) and yeast extract(1 g/L) was found to favor laccase production by Paecilomyces sp.S152 with an enzyme activity of 5064 U/L.The intra- and extra- cellular location of laccases were detected in the cells of Paecilomyces sp.S152.To improve cell permeability,Tween 80(1.5 g/L) was added to culture broth and an enhanced extracellular laccase activity was detected with the shortened fermentation period(6 days) and improved(946 U/(L·d).

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