利用色谱技术测定油茶籽油脂肪酸组成及维生素E质量浓度

Determination of Fatty Acid Composition and Vitamin E Content of Camellia Oleifera Oil by Chromatographic Technique

DOI：

中文关键词: 气相色谱法 高效液相色谱法 油茶籽油 脂肪酸组成 VE

英文关键词: gas chromatography HPLC camellia oleifera oil fatty acid composition VE

基金项目:国家“十一五”科技支撑计划项目(2009BADB1B09)

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中文摘要:

采用气相色谱法测定油茶籽油中脂肪酸组成,高效液相色谱法测定其维生素E含量。结果表明:初级压榨和精炼油茶籽油主要由4种脂肪酸组成,分别为棕榈酸(质量分数为7.68%和8.92%)、硬脂酸(质量分数为1.66%和1.78%)、油酸(质量分数为82.90%和81.09%)、亚油酸(质量分数为7.17%和7.22%);高效液相色谱法测定VE线性范围0.5～2.5μg(R2=0.999 3),平均回收率为98.05%,RSD为0.75%(n=5),初级压榨和精炼油茶籽油中维生素E含量分别为356.31mg/kg和107.07mg/kg。建立的色谱方法具有快速简便准确度高的特点。

英文摘要:

In this manuscript,fatty acid composition and Vitamin E content of Camellia oleifera oil were analyzed by Gas Chromatograph and HPLC respectively.The results show that: the primary crushing oil and refining oil are both contain 4 kinds of fatty acids mainly,which are palmic acid(7.68% and 8.92%),stearic acid(1.66% and 1.78%),oleic acid(82.90% and 81.90%),linoleic acid(7.17% and 7.22%);the linear range of VE was 0.5~2.5 μg(R2=0.999 3),and the mean recovery was 98.05%,RSD=0.75%(n=5).The VE content of primary crushing oil was 207mg/kg,which declined to 107.07mg/kg in refining oil.The chromatographic methods established in this text are fast,convenient and reliable.

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