产骨胶原蛋白酶菌种的筛选与鉴定

Isolation and Identification of a Strain with Producing Bone Collagenase

DOI：

中文关键词: 蜡样芽孢杆菌 骨胶原蛋白酶 分离 鉴定

英文关键词: Bacillus cereus bone collagenase isolation identification

基金项目:国家“十一五”科技支撑计划项目(2006BAD05A17)

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中文摘要:

从堆积骨骼的土样中经初筛、复筛和牛骨粉发酵实验,筛选出高产骨胶原蛋白酶的菌种MBL13(酶活36.80±1.93U/mL)。经形态学观察、生理生化特性、16SrDNA序列分析和傅里叶红外测定细胞结构,鉴定该菌株为蜡样芽孢杆菌(Bacillus cereus),并经牛骨胶原蛋白的酶解对比实验表明,MBL13所产的酶酶解骨胶原蛋白的能力强于标准品骨胶原酶I型(美国)。由于酶解后产生的多肽条带并不是连续的,说明发酵液中的胶原蛋白酶对牛骨胶原蛋白的酶解具有特定的酶切位点。

英文摘要:

In this study,a novel strain MBL13 was isolated from screened the soil in the animal bone factories through screening methods.It was found that the strain could secreted 36.80±1.93 U/mL collagenase in fermentation broth.Furthermore,the strain was identified as Bacillus cereus through morphology,physiology biochemistry characteristic,16S rDNA sequencing and Fourier Transform Infrared determination.the collagenase secreted from MBL13 exhibited higher hydrolysis capacity on bovine bone collagen than that of type I collagenase(United States).It was believed that the collagenase from MBL13 has the specific restriction sites due to the bands of peptides generated after digestion was not continuous.

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