VC混菌发酵中大菌不同胞外组分对小菌的影响

Effect of Different Components of Bacillus megaterium on Gluconobacter oxydansin Mix-Cultured of Vitamin C Fermentation

DOI：

中文关键词: 氧化葡萄糖酸杆菌 巨大芽孢杆菌 2-酮基-L-古龙酸 L山-梨糖脱氢酶

英文关键词: Gluconobacter oxydans Bacillus megaterium 2-Keto-L-gulonic acid L-sorbosone dehydrogenase

基金项目:沈阳市科学技术计划项目(F10-205-1-55);沈阳农业大学国家自然科学基金启动基金项目(20102002)

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中文摘要:

在维生素C(VC)二步混菌发酵中,巨大芽孢杆菌可显著促进氧化葡萄糖酸杆菌产VC前体2-酮基-L-古龙酸(2-KLG)。采用硫酸铵分级盐析沉淀、柱层析及电泳技术对巨大芽孢杆菌胞外液中的活性物质进行了分离纯化。通过测定氧化葡萄糖酸杆菌生长细胞转化L-山梨糖为2-KLG的生成量、产酸菌关键酶L-山梨糖脱氢酶(SDH)酶活性及产酸菌的活菌数,研究了VC两步发酵中大菌不同胞外组分对小菌的作用。结果表明:大菌胞外36 000和44 300两个组分蛋白质对小菌产酸、SDH酶活具有促进作用。

英文摘要:

In the process of vitamin C two-step fermentation,Bacillus megaterium(B.m) can enhance the ability of Gluconobacter oxydans(G.o) in convertingL-sorbose into 2-Keto-L-gulonic acid(2-KLG).In this study,isolated and purified active proteins were conducted by salting out of using ammonium sulfate fractionation,column chromatographyand electrophoresis technologyfrom the supernatant of B.megaterium.The effects of different components from the supernatant of B.megaterium on Gluconobacter oxydans growth and the ability of itsL-sorbose dehydrogenaseand yield of 2-KLGwere investigated,respectively.Among which the separated metabolites with molecularweightof 36 000 and 44 300 from B.megaterium significantly enhance the G.oxydansgrowth and 2-KLG production.

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