| 白藜芦醇对酪氨酸酶体外抑制活性的动力学研究
| Inhibitory Kinetics Study of Resveratrol on Tyrosinase Activity in Vitro
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| DOI：
| 中文关键词: 白藜芦醇 酪氨酸酶 抑制活性 酶促动力学
| 英文关键词: resveratrol tyrosinase inhibitory activity enzymatic kinetics
| 基金项目:中央高校基本科研业务费专项资金资助项目(TD2010-3、BLYX200935);第38批留学回国人员科研启动基金
| 作者
| 单位
| 杨阳
| 北京林业大学生物科学与技术学院,北京,100083
| 鲁军
| 中国食品发酵工业研究院,北京,100027
| 孙冰洁
| 北京林业大学生物科学与技术学院,北京,100083
| 王丹
| 北京林业大学生物科学与技术学院,北京,100083
| 尹晓燕
| 北京林业大学生物科学与技术学院,北京,100083
| 任迪峰
| 北京林业大学生物科学与技术学院,北京,100083
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| 中文摘要:
| 在30℃,pH 6.8的Na2HPO4-NaH2PO4的缓冲体系中,采用酶促动力学方法,研究了白藜芦醇对酪氨酸酶单酚酶和二酚酶的抑制作用。结果表明,白藜芦醇对酪氨酸酶、单酚酶和二酚酶均有抑制作用,对单酚酶抑制活性的IC50值(抑制率达到50%时的白藜芦醇质量浓度)约为5.1mg/mL,对二酚酶抑制活性的IC50值约为5.6 mg/mL。此外,白藜芦醇可延长单酚酶的迟滞效应,8 mg/mL的白藜芦醇能使迟滞时间从22 s延长至62 s,而对二酚酶则无此迟滞作用。Lineweav-er-Burk图分析表明,白藜芦醇对酪氨酸酶的抑制作用为混合型抑制,对游离酶的抑制常数(KI)和对酶-底物络合物的抑制常数(KIS)分别为3.4 mg/mL和35.98 mg/mL。
| 英文摘要:
| The inhibitory effect of resveratrol on the activity of monophenolase and diphenolase existing in tyrosinase was investigated by the enzymatic kinetic method at 30 ℃ in a Na2HPO4-NaH2PO4 buffer system(pH6.8).The results indicated that resveratrol could inhibit both the monophenolase and diphenolase activities of tyrosinase,and the IC50 values(resveratrol concentration corresponding to 50% inhibitory rate) were 5.1 mg/mL and 5.6 mg/mL,respectively.Furthermore,resveratrol could extent the lag time of monophenolase for oxidation of L-tyrosine,of which 8 mg/mL of resveratrol prolonged the lag time from 22 s to 62 s,while no such effect was observed on diphenolase.According to the Lineweaver-Burk plot,resveratrol was found to be a mixed inhibitor for the oxidation of L-DOPA,and the equilibrium constants for binding with free enzyme KI and with enzyme-substrate complex KIS were 3.4 mg/mL and 35.98 mg/mL,respectively.
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