酵母发酵过程中海藻糖的定性与定量测定

Qualitative and Quantitative Determination of Trehalose in Yeast Extract

DOI：

中文关键词: 海藻糖 DNS比色 蒽酮硫酸比色 纸层析 HPLC

英文关键词: trehalose DNS anthrone-sulfuric method paper chromatography HPLC

基金项目:

摘要点击次数: 170

全文下载次数: 1030

中文摘要:

应用纸层析对酵母提取液中海藻糖进行了定性分析,并应用蒽酮硫酸比色-DNS比色法对酵母提取液中海藻糖进行了定量测定,确定了海藻糖定性定量的条件。研究结果经与HPLC分析结果验证,表明该法对测定海藻糖含量具有较高的准确度和精密度,而且该法操作简单,易于掌握,比较适合在海藻糖产生菌筛选中应用。

英文摘要:

A method to determine trehalose in yeast extract using paper chromatography was investigated in this manuscript.Meanwhile,a process to determine the concentration of trehalose by Anthrone-sulphuric acid colorimetric method was developed.Compared to the results from HPLC,the paper chromatography method was identified as highly accurate and precise in the determination of trehalose.Therefore,the paper chromatography methods was proven to be easy operate and suitable for the screening of strains producing trehalose.

查看全文 查看/发表评论 下载PDF阅读器