超滤法分离富集谷朊粉抗氧化活性肽

Preparation and Enrichment Antioxidant Peptides from Wheat Gluten Hydrolysate using Ultrafiltration

DOI：10.3969/j.issn.1673-1689.2011.01.008

中文关键词: 谷朊粉 酶解产物 超滤 抗氧化活性 相对分子质量

英文关键词: gluten hydrolysate ultrafiltration antioxidant activity molecular weight

基金项目:国家863计划项目(2008AA10Z312)

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中文摘要:

为了研究谷朊粉酶解产物中发挥抗氧化活性的肽片段,采用截留不同相对分子质量的超滤膜对谷朊粉酶解产物进行初步分离富集,并通过测定各级多肽的亚铁离子螯合能力、对亚油酸的自氧化抑制能力以及对ABTS自由基的清除能力来评价其体外抗氧化能力。结果表明:抗氧化能力由弱到强依次为:大于10 000

英文摘要:

In order to study the active fraction of wheat gluten hydrolysates(WGH),WGH was preliminary separated and purified by using different MWCO of the ultrafiltration membrane.Different antioxidant assays in vitro such as ferrous ion-chelating activity,inhibition of linoleic acid emulsion peroxidation and 2,2’-azinobis(3-ethylbenothiazoline-6-sulphonic acid) diammonium salt(ABTS) radical scavenging activity,were employed to evaluate the antioxidant activities of the obtained wheat gluten hydrolysate.It was found that the antioxidant activity of the WGH can be arranged in the following order: over 10KD

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