一株产灵菌红素粘质沙雷氏菌的筛选、鉴定及发酵条件

Screening and Identification a Serratia marcescen Strain Producing Red-Pigment and Preliminary Study of the Fermentation Conditions

DOI：10.3969/j.issn.1673-1689.2012.10.002

中文关键词: 粘质沙雷氏菌(Serratia marcescens) 灵菌红素 筛选鉴定 发酵条件

英文关键词: Serratia marcescen prodigiosin screening and identification fermentation conditions

基金项目:国家863计划项目(2007AA02Z207);国家自然科学基金项目(30970056);中央高校基本科研业务费专项资金项目(JUSRP31001);教育部新世纪优秀人才计划项目(NCET-10-0459)

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中文摘要:

利用贫营养条件,从土壤样品中筛选到一株产红色素的菌株。经形态学、生理生化实验进行菌株初步鉴定,并经16S rDNA测序分析确定该菌株为粘质沙雷氏菌属,将其命名为:Serratiamarcescens JNB5-1。该菌株所产红色素经全波长扫描及LC-MS确定为灵菌红素。对Serratiamarcecens JNB5-1产灵菌红素做初步发酵研究,在蔗糖2 g/dL,牛肉膏1.5 g/dL,CaCl21 g/dL,脯氨酸0.75 g/dL,MgSO4.7H2O 0.02 g/dL,FeSO4.7H2O 0.006 g/dL的培养基中发酵72 h后,其发酵产量可达4.139 g/L。

英文摘要:

A strain producing red-pigment was newly isolated under poor nutrition conditions.It was identified by 16S rDNA sequence and systematic analysis,as well as general morphological and biochemical characteristics,The results show that 16S rDNA sequence of the strain suggesting that the strain is Serratia marcescens Species.And it was named Serratia marcescens JNB5-1.The red-pigment was identified as prodigiosin by wavelength scanning and LC-MS.preliminary fermentation conditions of strain Serratia marcescens JNB5-1 was studied.The results showed that the yield of prodigiosin was improved to 4.139 g/L after 72 h in the medium:sucrose 2%,beef extract 1.5%,CaCl2 1%,proline 0.75%,MgSO4·7H2O 0.02%,FeSO4·7H2O 0.006%.

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