牛奶中β-内酰胺和四环素类抗生素二联检测试纸条的初步研究

Study about Combo Detection Strip of β-Lactams and Tetracyclines in Milk

DOI：10.3969/j.issn.1673-1689.2012.07.017

中文关键词: 牛奶 β-内酰胺类抗生素 四环素类抗生素 胶体金 二联试纸条

英文关键词: milk β-lactams tetracyclines colloidalgold combo strip

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中文摘要:

抗生素越来越多地被应用于畜牧业中,其在乳制品中的残留问题也备受关注。乳品中残留抗生素不仅危害人体的健康,而且影响经济贸易的正常进行。为适应基层检测牛奶中抗生素残留的需求,作者采用胶体金免疫层析方法,建立了可以同时测定牛奶中的β-内酰胺类抗生素和四环素类抗生素的二联胶体金试纸条。该方法简单可靠,可同时检测牛奶中12种β-内酰胺类抗生素和4种四环素类抗生素,是一种值得推广的定性筛选方法,可作为液相色谱等定量检测仪器法的补充。

英文摘要:

Antibiotics were increasingly used in animal husbandry,the residue in the dairy products got more attention,the antibiotics residue in milk products not only harmful to human health,but also affect the economic trade.In order to adapt to the requirement of the antibiotic residues detection in milk,a combo strip that detect β-lactams and Tetracyclines in milk simultaneously was developt based on the colloidal gold immunochromatographic assay method in this study.The results present here demonstrated that 12 kinds of β-lactams and 4 kinds of tetracyclines antibiotics can be detected,and the method is simple,reliable.

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