微波-高压提取银杏叶黄酮工艺条件优化

Optimization of Microwave-High Pressure Assisted Extraction of Flavonids from Ginkgo biloba Leaves

DOI：10.3969/j.issn.1673-1689.2012.02.014

中文关键词: 银杏叶 黄酮 微波-高压 响应曲面法 大孔吸附树脂

英文关键词: Ginkgo biloba Leaves flavonoids microwave-high pressure response suface methodology macroporous adsorption resin

基金项目:安徽省年度科研计划项目(09020303078)

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中文摘要:

为了提高银杏叶中黄酮的得率,采用微波结合高压法联合提取,通过大孔吸附树脂分离、纯化黄酮。在单因素实验基础上,利用Box-Behnken组合设计研究pH,微波时间,压力3个因素对黄酮得率的影响。结果表明:黄酮提取的最佳工艺条件为:微波处理时间2.58min,压力为276.62MPa,pH为9.68,液料体积质量比30mL∶1g。在此条件下活性物质中黄酮的得率为5.14%。以吸附量和解吸率为指标对2种树脂进行对比,结果表明:AB-8是分离银杏叶黄酮的最佳树脂,静态饱和吸附量为16.2mg/g,吸附率81.1%,解吸率80.5%,纯化后银杏叶黄酮质量分数29.3%。

英文摘要:

To improve the flavonoids yield from Ginkgo biloba leaves,the combination of microwave and high pressure technique was employed to extract the flavonoids,which were isolated and purified by macroporous resins.On the basis of single factor experiment,Box-Behnken combination menthod was used to study the effect of the three factors(pH,microwave extraction time and pressure) on the flavonoids yield and the optimum conditions listed as follows: microwave extraction time 2.58 min,276.62 MPa pressure,9.68 pH and solid-liquid 30 mL∶1 g.Under the optimal condition,the yield rate of flavonoids compound was 5.14%.By comparing the two factors of adsorptive capacity and desorption of the two adsorption resins,results showed that: AB-8 was the optimal resin of the two kinds of macroporous resins to separate the flavones from Ginkgo biloba flavonoids.The static adsorptive capacity,adsorptive capability rate and desorption rate were 16.2 mg/g,81.1% and 80.5%,respectively.After purification,the purified flavonoids mass fraction was 29.3%.

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