色氨酸对慢性应激小鼠行为的影响

Effect of Tryptophan on Behavior of Chronically Stressed Mice

投稿时间：2012-07-26

DOI：

中文关键词: 色氨酸 慢性应激 5-羟色胺 动物行为 学习记忆能力

英文关键词: tryptophan chronic stress serotonin(5-HT) animal behavior learning and memorizing capability

基金项目:国家“十二五”科技支撑计划项目(2012BAD33B00);江南大学2011年大学生创新训练计划项目(1025210232113480)

摘要点击次数: 830

全文下载次数: 1215

中文摘要:

研究色氨酸对慢性应激小鼠行为的影响及与下丘脑5-HT的关系。将60只小鼠随机分为空白组(NC)、慢性应激模型组(CUS)、慢性应激模型+高剂量色氨酸(CUS-H)、慢性应激模型+低色氨酸(CUS-L),对CUS、CUS-H、CUS-L建立慢性应激模型,同时分别对CUS-H、CUS-L组灌胃高、低剂量的色氨酸,建模第5周开始进行旷场实验、悬尾实验、糖水消耗实验、强迫游泳实验及水迷宫实验等行为学实验,测定行为学指标,并采用ELISA法检测下丘脑5-HT含量。CUS-H组旷场实验得分显著高于CUS组与NC组(p

英文摘要:

Experiments were conducted to evaluate the effect of tryptophan on behavioral reactivity of stressed mice and its relation with hypothalamic 5-hydroxy tryptamine(5-HT).60 mice were randomly divided into 4 groups:NC group(saline administration),CUS group(saline administration and chronic stress stimulation),CUS-H group(high-dose tryptophan administration and chronic stress stimulation) and CUS-L group(low-dose tryptophan administration and chronic stress stimulation).A chronic stress model was established to CUS,CUS-H and CUS-L group.Meanwhile,different levels of tryptophan were administrated to CUS-H and CUS-L group.From the fifth week of establishing the model,open-field test,tail-suspension test,sugar preference test,forced swimming test and water maze were measured,and the hypothalamic serotonin level was assayed.The results are as follows:Compared to CUS and NC group,a notable increase(p

查看全文 查看/发表评论 下载PDF阅读器