五味子废渣中多糖的分离及体外抗氧化活性

Separation and Antioxidant Properties in Vitro of Polysaccharide from Schisandra chinensis Fruits Wastes

DOI：10.3969/j.issn.1673-1689.2013.02.009

中文关键词: 五味子 多糖 分离 自由基 抗氧化

英文关键词: Schisandra chinensis polysaccharide separation radicals antioxidant

基金项目:农业部公益性行业(农业)科技专项经费资助项目(201103037)

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中文摘要:

为了充分利用提取木脂素后的五味子废渣,提高其附加值,采用水提醇沉法提取五味子粗多糖,经聚酰胺柱层析及Sephacryl S-300 HR凝胶柱层析对其进行纯化,将得到的级分SCP-0采用Sephacryl S-300 HR凝胶柱层析法及凝胶渗透色谱法进行纯度鉴定;然后对SCP-0进行体外清除自由基活性测定。结果显示,其对.OH具有较好的清除效果,IC50为14.5 mg/mL;对DPPH.具有较好的清除效果,IC50为2.1 mg/mL;但对.O2-无明显清除效果;具有一定的抗油脂氧化能力,在低自由基浓度水平下效果更明显。

英文摘要:

The aim of this study is to find a way to reuse Schisandra chinensis fruits wastes after lignans extration.Polysaccharide tabled as SCP-0 was separated by hot-water extraction and ethanol precipitation,and then purified by polyamide column chromatography and Sephacryl S-300 HR column chromatography.After purity identification,the antioxidant activities of SCP-0 were investigated and the results showed a noticeable scavenging activity against hydroxyl radical and DPPH with IC50 of 14.5 mg/mL and 2.1 mg/mL respectively.However,superoxide anion scavenging activity of SCP-0 was hardly observed and certain ability of restraint on lard oxidation was observed.

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