傅立叶变换红外光谱技术对3种李斯特氏菌的快速分类鉴定

Rapid Differentiation and Identification of Three Species of Listeria by FT-IR Spectroscopy

DOI：10.3969/j.issn.1673-1689.2013.02.010

中文关键词: 傅立叶变换红外光谱 李斯特氏菌 主成分分析 分级聚类分析 分类 鉴定

英文关键词: Fourier transformation infrared spectroscopy Lisreria PCA HCA differentiation identification

基金项目:国家质量监督检验检疫总局科研项目(2010IK151;2012IK179)

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中文摘要:

为建立李斯特氏菌属内单核细胞增生李斯特氏菌、绵羊李斯特氏菌、英诺克李斯特氏菌3种菌的傅立叶变换红外(Fourier transform infrared,FT-IR)光谱数据库及FT-IT分类鉴定方法,作者应用FT-IR技术对3种李斯特氏菌进行指纹图谱数据采集,建立了3种李斯特氏菌的标准FT-IR导数谱数据库,同时结合化学计量学分析方法,建立了基于主成分分析(principalcomponent analysis,PCA)和分级聚类分析(hierarchical cluster analysis,HCA)两种聚类分析模型。结果表明,两种聚类模型均可成功将3种李斯特氏菌进行区分,FT-IR光谱数据库可用于FT-IR技术对3种可疑李斯特氏菌进行鉴定。FT-IR分析方法简便、快速、易操作,结果重现性好,是一种区分3种李斯特氏菌的有效方法。

英文摘要:

To establish a standard fourier transform infrared(FT-IR) spectral library and a FT-IR method of differentiation and identification of three species of Listeria,viz Listeria monocytogenes,Listeria iuanuii and Listeria innocua,FT-IR fingerprint absorption spectra of three species of Listeria were collected,and a standard spectral derivatives library was created.Combined with chemometrics methods,two cluster models of principal component analysis(PCA) and hierarchical cluster analysis(HCA) were established.It was found that three species of Listeria could be well differentiated and identified,and the standard spectral derivatives library created could be used to compare with those of target Listeria and identify them.As a rapid,easy-to-use and accurate technique,FT-IR spectroscopy is an effective tool to differentiate and identify three species of Listeria studied here.

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