重组人白细胞介素2-人血清白蛋白融合蛋白的液态稳定性

Stability of IL2-HSA Solution

DOI：10.3969/j.issn.1673-1689.2013.02.014

中文关键词: IL2-HSA pH 稳定性 液态 HPLC

英文关键词: IL2-HSA pH stability liquid HPLC

基金项目:江苏省产学研前瞻性联合研究项目(BY2009111)

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中文摘要:

考察了不同pH、缓冲液成分及不同保存环境下的IL2-HSA融合蛋白的稳定性,以SDS-PAGE和SE-HPLC作为考察手段并得出结论,在pH为4~6的缓冲盐体系中,蛋白质表现较稳定,当pH为5时,乙酸-乙酸钠缓冲体系较好的保持了蛋白质的稳定性。其后考察了蛋白质的辅料、光稳定性等因素对蛋白质的影响,在经受光照射后,蛋白质与对照样品未表现出明显区别。在乙酸盐提供的pH 5溶液中,辅料为氯化钠、贮存条件为4℃、贮存时间6个月后,蛋白质纯度仍保持在97%以上。

英文摘要:

The stability of interleukin2-HSA fusion protein was examined in different pH and buffer solution environments using SDS-PAGE and SE-HPLC.A pH-5 solution of Acetic acid Sodium acetate buffer is found to be more suitable for IL2-HSA to preserve stability.Several excipients was tested for the effect to protein stability under elevated storage temperature and photostability test was taken,the protein was proven to be stable throughout photo-stability test,after a 6month-long storage NaCl is considered to be a better excipient for IL2-HSA than its rivals.

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