食品和饲料中火鸡源性成分的实时荧光PCR检测方法

Identification of Turkey Derived Material in Food and Feed Using Real-Time PCR

DOI：10.3969/j.issn.1673-1689.2013.02.016

中文关键词: 食品 饲料 火鸡 实时荧光PCR方法 检测

英文关键词: food feed turkey derived material real-time PCR detection

基金项目:上海科委技术标准专项项目(06DZ05033);上海出入境检验检疫局科研项目(HK002-2012,沪科06-08)

摘要点击次数: 196

全文下载次数: 840

中文摘要:

为对产品真实性提供技术支撑,作者建立了食品和饲料中火鸡源性成分的检测方法。采用TaqMan探针实时荧光PCR方法,对火鸡源性成分特异性、灵敏度进行检测并进行实际应用。实验表明,建立的检测方法具有特异性和高灵敏度,检测限为0.001%。通过对市售食品和饲料样品的检测,建立的方法适用于食品和饲料中火鸡源性成分的检测。

英文摘要:

This work describes the development of a PCR system for the authentication of turkey derived material in food and feed.A real-time PCR method was established for detection of turkey derived material. The method was specific for turkey derived material,and the limit of detection was determined as 0.001%. The PCR procedure is suitable for the detection of turkey derived material in food and feed.

查看全文 查看/发表评论 下载PDF阅读器