白藜芦醇对不同时间高糖诱导HepG2细胞氧化损伤的保护作用

Protective Effects of Resveratrol Against Oxidative Damage Induced by High Glucose for Different Time in HepG2 Cell

DOI：10.3969/j.issn.1673-1689.2014.09.006

中文关键词: 白藜芦醇 HepG2细胞 高糖 氧化应激 作用机制

英文关键词: resveratrol, HepG2, high glucose, oxidative stress, mechanism

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中文摘要:

研究白藜芦醇对不同时间高糖诱导HepG2氧化损伤的保护作用。33 mmol/L高糖作为诱导剂，与不同浓度白藜芦醇（0.1、1、10 μmol/L）共同孵育24、48、72 h，采用MTT法检测其对损伤细胞存活率的影响，DCFH-DA荧光探针法检测细胞自由基水平，测定细胞超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-PX）、总抗氧化能力（T-AOC）水平，Real-time PCR测定Nrf2、HO-1 mRNA表达水平，探讨白藜芦醇对高糖损伤细胞的保护作用及可能机制。结果表明，白藜芦醇与高糖共同作用48 h以上时，各浓度组均能显著提高损伤细胞存活率，显著降低细胞自由基水平（p

英文摘要:

To investigate the protective effects of resveratrol against oxidative damage induced by high glucose for different time in HepG2 cell. The cells were treated with 33 mmol/L glucose to induce oxidative damage and with different concentrations of Res 0.1, 1, 10 μmol/L for 24, 48, and 72 h. The cell viability was measured by MTT assay, DCFH-DA was used as fluorescence probe for observing ROS level in cells, superoxide dismutase(SOD), malondialdehyde(MDA), glutathione peroxidase(GSH-PX), total antioxidative capacity(T-AOC) were determined by using commercially available kits. Real-time PCR was used to determine the relative mRNA expression of nuclear factor-E2-related factor(Nrf2) and hemeoxygenase-1(HO-1). Results show that compared with high glucose damage group, after 48h, cell viability of Res groups was significantly increased(p

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