温度对高顺式占比番茄红素稳定性影响研究

Study on the Influence of Temperature on the Stability of High Proportion of Z-Lycopene Isomers

DOI：10.3969/j.issn.1673-1689.2014.07.007

中文关键词: 番茄红素 稳定性 异构化 降解动力学 活化能

英文关键词: lycopene, stability, isomerization, degradation kinetics, activation energy

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中文摘要:

相比全反式，顺式番茄红素异构体具有更高的生物活性和生物利用率，但易氧化降解及向反式构型的回复。作者以YMC Carotenoid C30-HPLC为检测系统研究温度对其稳定性影响。结果表明以乙酸乙酯为介质，热回流-重结晶联用技术制备了顺式异构体占比达78%~85%的番茄红素产品。贮存稳定性试验表明，温度越低，各异构体降解速率常数的绝对值（k）越小，表明其降解越慢、贮藏越稳定。同时模型参数表明，等温条件下，kAll-E＜k9-Z＜k13-Z。9-Z、13-Z、All-E 和All-Z异构体在贮藏过程中表现出来的活化能分别为46.42，29.19，56.94，32.45 kJ/mol。所以3种主要番茄红素异构体中，稳定性由高到低依次为：All-E＞9Z＞13Z。高顺式占比产品表现出总的活化能为33.76 kJ/mol。

英文摘要:

Compared to All-E lycopene, Z-isomers had higher biological activity and bioavailability, but tending to oxidative degradation and conversion to E-isomer. In the paper, YMC Carotenoid C30-HPLC system were used to detect the influence of temperature on isomers' stability. The results showed that the proportion of cis-isomer lycopene could reach 78-85% in ethyl acetate by combining refluxing-crystal techniques. Storage stability test showed that the lower the temperature was, the smaller of degradation rate constant(k) of lycopene isomers was, indicating that the slower rate of degradation, more stable the storage. While the model parameters indicated that under isothermal conditions, kAll-E＜k9-Z＜k13-Z. The activation energies of 9-Z, 13-Z, All-E and All-Z isomers were 46.42 kJ/mol, 29.19 kJ/mol, 56.94 kJ/mol and 32.45 kJ/mol during storage respectively. So stability order of three major lycopene isomers was: All-E＞9Z＞13Z. And the activation energy of high proportion of Z-lycopene isomer product was 33.76 kJ/mol.

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