150 healthy Kunming mice were randomly divided into three batches(5 groups each batch) for loaded swimming test and biochemical analysis. We divided Maca into low, medium and high dose groups(0.03 g/ kg.bw.d, 0.30 g/kg.bw.d, 1.00 g/kg.bw.d), and the control group(saline), positive control group(YiKang capsules for 0.30 g/ kg.bw.d) respectively. Mice were administered ig daily for 30 d, then the loaded-swimming time, blood urea nitrogen, blood lactate, hemoglobin, lactate dehydrogenase and hepatic glycogen of them were measured．In addition, active ingredients related to anti-fatigue in Maca such as amino acids, polysaccharides, fructose and glucosinolates were quantitative detected through colorimetry or HPLC. Results show that, Maca is rich in amino acids(158.10 mg/g), total flavonoids(20.83 mg/g), polysaccharides(37.16 mg/g), fructose(31.27 mg/g), total alkaloids(4.22 mg/g), mustard oil glycosides(1.08 mg/g) and other ingredients. Animal testing results show that, the high, middle, low dose groups could all significantly prolong the time of loading swimming, increase the hemoglobin, lactate dehydrogenase and hepatic glycogen, decrease blood urea nitrogen and blood lactate in mice compared with negative control. Maca is rich in a variety of active ingredients related to anti-fatigue, and it has significant anti-fatigue function in healthy mice, may be ideal raw material for anti-fatigue functional foods.
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