姬菇菇脚对红曲霉菌丝生长和产色素的影响

Effect of P. cornucopiae Stembase on Mycelium Growth and Pigment Yield of Monascus

DOI：10.3969/j.issn.1673-1689.2014.05.013

中文关键词: 姬菇菇脚 紫红曲霉MR02 菌丝生物量 红曲色素

英文关键词: Pleurotus cornucopiae stembase, Monascus purpureous MR02, mycelium biomass, monascus pigment

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中文摘要:

为调查以姬菇菇脚为底物进行液态发酵生产天然红曲色素的可行性，作者采用平板培养和液态发酵技术，以平板菌落直径、发酵液菌丝体生物量和色素产量为指标，评估姬菇菇脚对紫红曲霉MR02菌丝生长和产色素能力的影响。结果表明，普通培养基添加姬菇菇脚能促进紫红曲霉MR02菌丝体的生长和色素产量的提高；以不同配比的菇脚和大米粉为底物进行培养，在单一菇脚为底物的培养液中测得一定量的菌丝体和红曲色素；以菇脚为底物培养紫红曲霉MR02，其生长特性符合多细胞生物的生长曲线规律；以不同质量分数的姬菇菇脚为底物发酵，菇脚质量分数为12%，培养到第144小时时，菌丝体生物量为5.52 g/L，培养到第216小时时，红曲色素产量为72.92 U/mL。因此，以紫红曲霉MR02为出发菌株，利用姬菇菇脚为底物发酵生产红曲色素是可行的。

英文摘要:

In order to investigate the feasibility of Pleurotus cornucopiae stembase as a substrate for producing natural red pigment through the liquid fermentation, we evaluated the effects of P. cornucopiae stembase on mycelium growth and pigment yield of Monascus purpureous MR02 through measuring the colony diameter, mycelium biomass and pigment values during plate culture and liquid fermentation. The results showed that general medium supplemented with the P. cornucopiae stembase powder can promote the mycelium growth and pigment production of M. purpureous MR02; through the cultivation of the P. cornucopiae stembase and rice powder as substrate, a certain amount of mycelium biomass and red pigment was tested in the fermentation broth of only P. cornucopiae stembase as a substrate; and culvivated by P. cornucopiae stembase as a substrate, we found that the growth curves of M. purpureous MR02 were in accordance with the growth curves of multi-cellular microorganism; through the cultures of M. purpureous MR02 under different concentrations of P. cornucopiae stembase as the substrate, mycelium biomass of 5.52 g/L and pigment values of 72.92 U/mL were obtained during the liquid fermentation of 144 h and 216 h by using 12% of P. cornucopiae stembase, respectively. Therefore, it is feasible to exploit the P. cornucopiae stembase as a substrate for producing natural red pigment by M. purpureous MR02.

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