基于形态特征和ITS序列分析秦巴山区黑木耳主要栽培种的亲缘关系

Analysis of the Genetic Relationships of Main Auricularia auricular Cultispecies in Qinba Mountainous Area Based on ITS Sequence

DOI：10.3969/j.issn.1673-1689.2014.05.014

中文关键词: 黑木耳 菌丝 生长速度 ITS序列

英文关键词: Auricularia auricular, mycelia, growth rate, ITS

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中文摘要:

采用CTAB法提取黑木耳菌株的DNA，进行PCR扩增，扩增产物进行测序。耳2和标准菌株Auricularia auricula-judae HF-8（GQ168492）聚类在同一分支上；耳913和标准菌株Auricularia auricula-judae YBS-7（GQ168503）聚类在同一分支上，且神农A8与新科、耳2、耳913亲缘关系相对较远。耳97-2和标准菌株Auricularia auricula-judae NW482（EU520194）聚类在同一分支上；耳268和标准菌株Auricularia auricula-judae NW491（EU520160）聚类在同一分支上，且秦单4#与耳97-2、耳268亲缘关系相对较远。新科、耳2、耳913、神农A8此4个菌种亲缘关系比较近；耳268、秦单4#、耳97-2等3个菌种亲缘关系较近。通过黑木耳菌株的形态观察及ITS序列分析，得到两株亲缘关系较远的优势菌株秦单4#和神农A8。

英文摘要:

Analyzing the genetic relationships of the main Auricularia auricular cultispecies in Qinba mountainous area by ITS sequence so as to lay a theoretical foundation to genetics and breeding. Using CTAB method to extract genomic DNA from the bacterial strain, Auricularia auricular, and amplifying and sequencing PCR products; Auricularia auricula-judae 2 and Auricularia auricula-judae HF-8(GQ168492) were clustered in the same branch, Auricularia auricula-judae 913 and Auricularia auricula-judae YBS-7(GQ168503) were clustered in the same branch, but the genetic relationship between Auricularia auricula-judae Shennong A8 and the Auricularia auricula-judae 2, Auricularia auricula-judae 913 were relatively alienative. Auricularia auricula-judae 97-2, Auricularia auricula-judae NW482(EU520194), the Auricularia auricula-judae 268 and Auricularia auricula-judae NW491 (EU520160) were clustered in the same branch. However the genetic relationships among Auricularia auricula-judae Qindan 4, Auricularia auricula-judae 97-2, Auricularia auricula-judae 268 were relatively alienative. The genetic relationships among Auricularia auricula-judae Xinke, Auricularia auricula-judae 2, Auricularia auricula-judae 913 and Auricularia auricula-judae Shennong A8 were close. However, the relationships between Auricularia auricula-judae 268, Auricularia auricula-judae Qindan 4# and Auricularia auricula-judae 97-2 were quite far. Through the morphologic and ITS analysis of Auricularia auricular strains, two dominant strains, Auricularia auricula-judae Qindan 4# and Auricularia auricula-judae Shennong A8, have alienative relationship. Our findings established a solid foundation to genetics and breeding.

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