高效毛细管电泳法测定食品中5种甜味剂含量

Simultaneous Determination of 5 Sweeteners in Food by HPCE

DOI：10.3969/j.issn.1673-1689.2014.03.014

中文关键词: 高效毛细管电泳 合成甜味剂 定量分析

英文关键词: high performance capillary electrophoresis(HPCE) artificial sweeteners quantitative analysis

基金项目:安徽省自然科学基金项目(1308085QB24);安徽省高校省级优秀青年人才基金重点项目(2013SQRL051ZD);安徽省生化药物工程技术研究中心开放课题(BYEC1202,BYEC1301)

摘要点击次数: 310

全文下载次数: 953

中文摘要:

采用高效毛细管电泳-紫外可见检测法同时测定食品中5种人工合成甜味剂纽甜、阿斯巴甜、甜蜜素、糖精钠、安赛蜜的含量。内壁未涂层熔融石英毛细管(50μm×75 cm,有效长度60 cm),毛细管温度为25℃;缓冲液为0.2 mol/L硼酸盐(pH=8.29),分离电压+23 kV,进样压力3.45 kPa,进样时间3.0 s,UV-Vis检测器检测波长200 nm。结果显示:5种甜味剂线性回归方程相关系数r在0.999 21~0.999 89之间,定量限在2.2~552.6 mg/L之间,样品加标回收率在96.29%~102.31%范围内,相对标准偏差在3.81%~6.40%范围内。表明该方法简便、灵敏、快速、可靠、重现性好,能用于食品中5种甜味剂的同时测定。

英文摘要:

The method of simultaneous determination of five kinds of artificial sweetener neotame, aspartame,sodium cyclamate,sodium saccharin,acesulfame content was established by HPCE with UV-Vis detector in this manuscript. The fused silica capillary with uncoated inner wall(50 μm×75 cm,effective length 60 cm) was used,and the capillary was kept at 25 ℃. 0.2 mol/L borate(pH= 8.29) was adopted as electrophoresis buffer at +23 kV separation voltage. The sample was injected under 3.45 kPa pressure for 3.0 seconds,and detection wavelength being set at 200 nm. The linear regression equation correlation coefficient r for five kinds of sweeteners was between 0.999 21 and 0.999 89. The detection limit of five kinds of sweeteners was in the range of 2.2~552.6 mg/L. The spiked recoveries were within the range from 96.29% to 102.31%,and the relative standard deviation was kept between 3.81% and 6.40%. Conclusion The method is simple,sensitive,rapid, reliable and well reproducible,it can be used for the determination of five sweeteners in food simultaneously.

查看全文 查看/发表评论 下载PDF阅读器