紫椴花多糖的微波提取及体外抗氧化活性

Microwave-Assisted Extraction and Antioxidant Activity in Vitro of Polysaccharides from Tilia amurensis Rupr. Flowers

DOI：10.3969/j.issn.1673-1689.2014.03.015

中文关键词: 紫椴 多糖 提取 体外抗氧化

英文关键词: Tilia amurensis Rupr polysaccharides extraction antioxidant activity in vitro

基金项目:哈尔滨市科技创新人才研究专项资金(2013RFXXJ027)

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中文摘要:

以干燥紫椴花为原料,微波提取水溶性多糖,考察了微波时间、微波功率、料液比3个因素对多糖得率的影响,通过响应面法对紫椴花多糖微波提取工艺进行了优化,根据二次回归模型得到的最佳工艺条件为:微波时间9 min,微波功率585 W,料液比1∶67(g/mL),在此条件下,紫椴花多糖理论得率为12.57%。理化性质实验及红外光谱分析表明,紫椴花多糖具有多糖的通性。以抗坏血酸(VC)为对照品,测定了紫椴花粗多糖的总还原能力、清除1,1-二苯基-2-三硝基苯肼(DPPH)和羟自由基(·OH)的能力,及对亚铁离子(Fe2+)诱发的脂质过氧化的抑制作用,对紫椴花粗多糖的体外抗氧化活性进行了评价。结果表明:紫椴花粗多糖具有一定的还原能力、清除自由基能力,及抗脂质过氧化能力,其抗氧化活性弱于相同浓度的抗坏血酸(VC)。

英文摘要:

In this manuscript,water soluble polysaccharides were extracted from Tilia amurensis Rupr. flowers by microwave-assisted method was developed. The effect of microwave time,microwave power and ratio of raw material to liquid on the yield of polysaccharides was investigated and the microwave-assisted extraction process was optimized by response surface method. According to quadratic regression model,the optimum extraction parameters was 9 min,585 W and 1∶67(g∶mL). Under this optimum conditions,the yield of polysaccharides in theory was achieved at 12.57%.The physical and chemical properties experiment and infrared spectrum analysis indicated that polysaccharides extracted from Tilia amurensis Rupr. flowers presented typical properties of polysaccharides. The antioxidant activity in vitro of polysaccharides from Tilia amurensis Rupr. was evaluated by determining the reducing power,scavenging capacities on 1,1-diphenyl-2-picrylhydrazyl radical and hydroxyl free radical and the inhibition effect on lipid peroxidation induced by ferrous ion. The results showed that polysaccharides from Tilia amurensis Rupr. had certain reducing power,scavenging capacities on free radical and inhibition effect on lipid peroxidation,which were appreciably weaker than ascorbic acid(VC).

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