油菜蜂花粉多糖的分离鉴定及免疫活性研究

Isolate, Identify and the Immune Improving Activity of Polysaccharides from Rape Bee Pollen

DOI：10.3969/j.issn.1673-1689.2015.10.006

中文关键词: 油菜蜂花粉水提物 免疫活性 分离 多糖

英文关键词: rape bee pollen,immune activity,isolation,polysaccharide

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中文摘要:

油菜蜂花粉粉碎后经体积分数95%乙醇脱脂，过滤，滤渣用去离子水60 ℃提取，再用体积分数95%乙醇沉淀提取液，得到油菜蜂花粉水提醇沉物。利用DEAE-纤维素柱、HW-55F色谱柱及Sephacryl色谱柱 、Sepharose色谱柱对油菜蜂花粉水提醇沉物进行分离，得到一个主要的多糖类化合物。经Sephadex G-100色谱柱及紫外光谱分析对该化合物进行纯度鉴定，再通过核磁方法对其进行1H-NMR、13C-NMR和135DEPT-NMR分析，得该化合物结构为HOCH2-[CHOH]4-COOH。给免疫抑制小鼠灌胃花粉多糖，实验结果表明：油菜蜂花粉多糖能显著提高免疫抑制小鼠的胸腺指数（P

英文摘要:

Rape bee pollen was powered and extracted by 95% ethanol under reflux，then extracting the filer residue by deionized water under 60 ℃. Adding 95% ethanol into the extract，rested and layered，then acquired the ethanol sediment of rape bee pollen. Isolating the ethanol sediment with DEAE-cellulose column，HW-55F chromatographic column，Sephacryl chromatographic column and Sepharose chromatographic column，acquired one main polysaccharide compound. Identifing the purity of this compound by Sephadex G-100 chromatographic column and ultraviolet spectral analysis，then using nuclear magnetic mathod to analyse the 1H-NMR、13C-NMR and 135DEPT-NMR of this compound，obtained its structural formula：HOCH2-[CHOH]4-COOH. Irrigating the immune suppression mice with this component of rape bee pollen，results showed that this component of rape bee pollen could improve the thymus index（P

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