生物素间接竞争酶联免疫方法的建立

An Indirect Competitive Enzyme-Linked Immunosorbent Method for the Detection of Biotin

DOI：10.3969/j.issn.1673-1689.2015.10.007

中文关键词: 乳制品 生物素 间接竞争酶联吸附测定法

英文关键词: dairy products,biotin,indirect competitive enzyme-linked immunosorbent assay（Ic-ELISA）

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中文摘要:

利用不同方法合成的生物素免疫原免疫小鼠，利用所得到的单克隆抗体建立针对生物素的间接竞争酶联吸附测定方法。所建立的间接竞争酶联吸附测定方法对生物素具有良好的特异性与较高的灵敏度，其对生物素半抑制率质量浓度为2.01 ng/mL，最低检测限为0.32 ng/mL，并且对液态奶、奶粉等样品中的生物素含量具有准确的检出性。与国标相比，所建立的间接竞争酶联吸附测定方法具有结果稳定，操作方便和高灵敏度高特异性等优点，对实际样品的检测具有重要意义。

英文摘要:

Different kinds of biotin immunogens were synthesized for mice immunization and the obtained monoclone antibody was used for the development of indirect competitive enzyme-linked immunosorbent assay. With the method of high specificity and sensitivity，the half inhibition concentration of biotin was 2.01 ng/mL and the detection limit was 0.32 ng/mL，and the biotin content in liquid milk and milk powder samples could be accurately detected. Compared with the national standard，the method was sufficiently stable，convenient，sensitive and accurate for the analysis of biotin in real samples.

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