响应面优化超声-微波协同提取石榴叶中总黄酮工艺的研究
Optimization of Ultrasonic-Microwave Synergistic Extraction of Total Flavonoids from Leaves of Punica granatum L. by Response Surface Methodology
DOI：10.3969/j.issn.1673-1689.2015.07.012
中文关键词: 超声-微波协同萃取 石榴叶 总黄酮 响应面法
英文关键词: ultrasonic-microwave synergistic extraction, leaves of Punica granatum L., total flavonoids, response surface methodology
基金项目:
作者
单位
滕井通
淮北师范大学 生命科学学院，安徽 淮北 235000
资源植物生物学安徽省重点实验室，安徽 淮北 235000
淮北普豪生物科技有限公司，安徽 濉溪 234100
詹小龙
宫坤
薛建平
段永波
宋迎春
盛玮
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中文摘要:
为确定石榴叶中总黄酮提取的最佳工艺，在单因素试验的基础上，应用响应面法优化石榴叶中总黄酮的提取条件。结果表明:微波功率、提取时间和料液比对黄酮提取率影响极显著；最佳工艺为:乙醇体积分数71.5%(v/v)，微波功率662 W，提取时间262 s，液料质量体积比33.5 g/mL，在此条件下提取1 次，石榴叶中总黄酮的提取率为89.21%。
英文摘要:
To optimize the extraction technology of total flavonoids from Leaves of Punica granatum L.，response surface methodology was applied on the basis of single factor tests. The results showed that microwave power，extraction time and liquid-to-material ratio had extremely significant effect on the extraction rate of total flavonoids. Ethanol concentration of 71.5%(v/v)，microwave power of 662 W，extraction time of 242 s and solid/liquid ratio of 33.5(g/mL) were found optimum. Under the optimized conditions，the extraction rate of total flavonoids from Leaves of Punica granatum L. was 89.21% by extracting once.
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